Repair abilities of mouse autologous adipose-derived stem cells and ShakeGel™3D complex local injection with intrauterine adhesion by BMP7-Smad5 signaling pathway activation

Abstract Background The objective was to explore the therapeutic effect of autologous adipose-derived stem cells (ADSCs) combined with ShakeGel™3D transplantation activate BMP7-Smad5 signaling pathway treat intrauterine adhesions (IUA). Methods Autologous ADSCs were isolated and then merged ShakeGel™3D. IUA model established by mechanical injury. third generation injected directly into uterus in combination After 7 days treatment, endometrial morphology, number glands, fibrosis area, biomarker analysis RT-PCR IHC examined. BMP7 phosphorylation Smad5 also detected, recovery infertility function treated mice evaluated. Results Fluorescence-activated cell sorting (FACS) showed that expressed CD105 (99.1%), CD29 (99.6%), CD73 (98.9%). could still maintain a good growth state Histological examination revealed glands increased significantly, area decreased. At same time, expression + Gel group significantly upregulated, final reproductive this partly recovered. Conclusions can be used functionality create viable three-dimensional environment. promotes damaged tissue increasing signal transduction, resulting endometrium thickening, decreased fibrosis, leading restoration partial fertility.